Where is Twylia?

Who is Jane Doe?

"We are relieved of course," said Jennifer Kitt, on the news that a second DNA test has shown that the body of a young woman discovered in Boulder, Colo., in 1954 was not her great-aunt, Twylia May Embrey.

Boulder County Sheriff's Office detective Steve Ainsworth called Kitt late Tuesday night with the news.

"It was strange," Kitt said.

Kitt has spent the past two years trying to find out what happened to her relative for her grandmother, Twylia's sister, Mildred "Midge" Garner of Wellfleet.

"I called Grandma last night and told her," Kitt said. "She was very glad it wasn't our Twylia, but was sad that there is another family who doesn't have any answers."

Kitt traveled to Boulder last month to be interviewed for the television program "America's Most Wanted," which is planning a show on the case of the unidentified Boulder Jane Doe.

The efforts to find out what happened to her relative has led Kitt on an emotional journey with as many twists and turns as the Boulder Canyon road where Jane Doe's body was discovered by two University of Colorado students in April 1954.

A story in The Telegraph about the Boulder case led Kitt to Silvia Pettem, the Boulder historian who has resurrected Jane Doe's case. Pettem persuaded the Boulder Sheriff's Office to take a more active look at the case, which had faded from the community's memory. Kitt contacted Pettem with the story of her great-aunt.

Twylia May Embrey disappeared from North Platte sometime in 1953.

Initially, investigators ruled out Twylia as Jane Doe. A photo of Twylia was superimposed over Jane Doe's skull, and the two did not match. After a forensic sculpture was made of Jane Doe's skull, sculptor Frank Bender told investigators and the family that there were signs that the skull might match Embrey after all. Based on Bender's recommendations, DNA was taken from a member of Twylia's family and sent off for comparison to Boulder Jane Doe.

In July, Boulder County Sheriff's office announced the DNA did not match.

According to Lt. Phil West, the original lab had conducted three comparison tests on DNA from Jane Doe and Garner.

"We were told one was positive and two were negative," West said. "So according to their procedures they called it 'no match.' We consulted with experts in the field of comparison who has suggested to assure ourselves that the comparison was in fact negative, we have another lab conduct a second test."

Kitt said when she contacted her grandmother with the news that the second test had been no match to Twylia, Garner asked her to promise she wouldn't give up.

"I told her that I wouldn't," Kitt said. "But for the first time, she said she felt, 'deep down in her gizzard,' that Twylia is dead."

Kitt has an ally in Pettem, who has promised to help continue the search for Twylia while she continues to try and identify Jane Doe.

"We are back to where we were before," Pettem said from her home west of Boulder Wednesday morning. "I am still very committed to helping Twylia's family to find her. I will follow this through to the end."

Pettem said she was waiting for word from America's Most Wanted producers about the program on Boulder Jane Doe.

According to Kitt, who contacted AMW producer Fred Peabody, the program intends to include the search for Twylia in the program on Jane Doe.

"It will get her face out there to the public," Kitt said. "It will reach a nationwide audience, and hopefully, someone out there will recognize her and let us know what happened to her."

Among the piles of paperwork Kitt has amassed in her search is a bill of sale, dated 1959, detailing Twylia's parents' sale of their farm and all their possessions.

"They sold everything and bought a rickety old car with a mattress in the back," Kitt said.

"They never had a home again until they went to the nursing home."

Charles and Iva "Addie" Embrey traveled all over the country, searching for their missing daughter, the second youngest of eight children.

"When they ran out of money, they would come home for a while and live with Grandma until they could go out looking again," Kitt said.

Despite this latest setback, someone is still out there, looking for Twylia.